Bioinformatics: What Does the Sequence Mean?
Bioinformatics: What Does the Sequence Mean? What is bioinformatics? As you might imagine, sequencing entire genomes generates a huge volume …
Recombinant DNA Technology
Cas9 Nuclease for Genome Editing
Cas9 Nuclease Is a Precise Tool for Genome Editing Introduction Cas9 genome editing has rapidly become one of the most …
Tools of Genetic Engineering to Make Genome Modifications
Tools of Genetic Engineering to Make Genome Modifications Introduction The field of genetic engineering includes many techniques and depends on …
Microbial Identification and Strain Typing Using Molecular Techniques
Microbial Identification and Strain Typing Using Molecular Techniques Introduction With the rise of large numbers of antibiotic resistance isolates of …
Reverse Transcription Polymerase Chain Reaction (RT-PCR)
What is RT-PCR? The most sensitive technique available for detecting and quantifying messenger RNA (mRNA, or transcript) is RT-PCR, also …
Transposable Elements
Transposable Elements As more and more genomes have been sequenced and annotated, it has become increasingly apparent that the genomes …
Whole-Genome Shotgun Sequencing: overview, steps and achievements
Whole-Genome Shotgun Sequencing: overview, steps and achievements In the past many virus genomes have been sequenced, but bacterial genome is …
Plasmid: Properties, Types, Replication and Organization
Plasmid: Properties, Types, Replication and Organization Properties of Plasmid Many prokaryotes contain extrachromosomal DNA molecules, The genetic material present in …
Vector: properties, types and characteristics
Vector: properties, types and characteristics Content Introduction Properties of good vector Types of vector Plasmid vector Cosmids Phagemids Phage vector …
Southern Blotting
Southern Blotting Another problem early biotechnologists faced was the inability to distinguish the fragment of DNA possessing the gene of interest …