Bioinformatics: What Does the Sequence Mean?
Bioinformatics: What Does the Sequence Mean? What is bioinformatics? As you might imagine, sequencing entire genomes generates a huge volume of information. The field of …
Recombinant DNA Technology
Cas9 Nuclease for Genome Editing
Cas9 Nuclease Is a Precise Tool for Genome Editing Introduction Cas9 genome editing has rapidly become one of the most widely used tools for altering …
Tools of Genetic Engineering to Make Genome Modifications
Tools of Genetic Engineering to Make Genome Modifications Introduction The field of genetic engineering includes many techniques and depends on a group of tools to …
Microbial Identification and Strain Typing Using Molecular Techniques
Microbial Identification and Strain Typing Using Molecular Techniques Introduction With the rise of large numbers of antibiotic resistance isolates of many microorganisms, increases in the …
Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Reverse Transcription Polymerase Chain Reaction (RT-PCR) What is RT-PCR? The most sensitive technique available for detecting and quantifying messenger RNA (mRNA, or transcript) is RT-PCR, …
Transposable Elements
Transposable Elements As more and more genomes have been sequenced and annotated, it has become increasingly apparent that the genomes of all organisms are rife …
Whole-Genome Shotgun Sequencing: overview, steps and achievements
Whole-Genome Shotgun Sequencing: overview, steps and achievements In the past many virus genomes have been sequenced, but bacterial genome is not possible to be sequence. …
Plasmid: Properties, Types, Replication and Organization
Plasmid: Properties, Types, Replication and Organization Properties of Plasmid Many prokaryotes contain extrachromosomal DNA molecules, The genetic material present in the nucleoid is known as …
Vector: properties, types and characteristics
Vector: properties, types and characteristics Content Introduction Properties of good vector Types of vector Plasmid vector Cosmids Phagemids Phage vector or Bacteriophage vector Artificial chromosome …
Southern Blotting
Southern Blotting Another problem early biotechnologists faced was the inability to distinguish the fragment of DNA possessing the gene of interest from the numerous chromosomal fragments …