Bioinformatics: What Does the Sequence Mean?
Introduction Bioinformatics is an interdisciplinary field that combines biology, computer science, mathematics, and statistics to analyze and interpret biological data. …
Recombinant DNA Technology
Recombinant DNA Technology (also called genetic engineering) is a set of techniques used to combine DNA from different sources to create new genetic combinations that are valuable for research, medicine, agriculture, and industry. Recombinant DNA technology involves the manipulation and recombination of DNA molecules to produce new genetic material that can be introduced into an organism to change its traits or produce useful products.
Cas9 Nuclease for Genome Editing
Introduction Cas9 genome editing has rapidly become one of the most widely used tools for altering genomes in vivo. Cas9 …
Tools of Genetic Engineering to Make Genome Modifications
Introduction The field of genetic engineering includes many techniques and depends on a group of tools to manipulate DNA. Two …
Microbial Identification and Strain Typing Using Molecular Techniques
Introduction With the rise of large numbers of antibiotic resistance isolates of many microorganisms, increases in the rates of toxin-producing …
Reverse Transcription Polymerase Chain Reaction (RT-PCR)
What is RT-PCR? The most sensitive technique available for detecting and quantifying messenger RNA (mRNA, or transcript) is RT-PCR, also …
Transposable Elements
Introduction Transposons, also known as “jumping genes,” are DNA sequences that can move from one location to another within a …
Whole-Genome Shotgun Sequencing: overview, steps and achievements
Introduction Whole-Genome Shotgun Sequencing (WGS) is a powerful method used to determine the entire DNA sequence of an organism’s genome …
Plasmid: Properties, Types, Replication and Organization
Introduction A plasmid is a small, circular, double-stranded DNA molecule that exists independently of chromosomal DNA in bacteria and some …
Vector: properties, types and characteristics
Introduction A vector is a carrier DNA molecule that can replicate within a host cell and is used to transfer …
Southern Blotting
Southern Blotting Another problem early biotechnologists faced was the inability to distinguish the fragment of DNA possessing the gene of interest …