Free Online Microbiology Calculator

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MICROBIOLOGY NOTES · LAB TOOLS

Microbiology Notes Lab Calculator

Professional laboratory calculation tools

FREE LAB TOOL
PLATE COUNT METHOD

CFU/mL Calculator

Calculate viable microbial concentration in liquid samples.

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Average Colony Count: 87.5
FINAL RESULT 8.75 × 10⁶ CFU/mL Average of two plate counts.

Formula: CFU/mL = Average colonies ÷ (Dilution fraction × Volume plated).

SOLID SAMPLE ENUMERATION

CFU/g Calculator

Calculate microbial concentration per gram of sample.

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Average Colony Count: 78
FINAL RESULT 7.8 × 10⁵ CFU/g Average of two plate counts.

Formula: CFU/g = Average colonies ÷ (Subsequent dilution fraction × Volume plated × Initial homogenate concentration in g/mL).

STEPWISE DILUTION

Serial Dilution Calculator

Calculate cumulative dilution across repeated transfer steps.

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FINAL DILUTION 10⁻⁶ Six successive 1:10 dilutions.
TubeStep DilutionCumulative Dilution
MICROBIAL INACTIVATION

Log Reduction Calculator

Calculate microbial reduction after treatment.

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LOG REDUCTION 3.00 log₁₀ 99.9% reduction.
REPLICATE PLATES

Average Colony Count

Calculate the arithmetic mean of colony counts.

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AVERAGE COLONIES 85 Three observations.
DILUTION NOTATION

Dilution Factor Converter

Convert dilution exponent and reciprocal factor.

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DILUTION 10⁻⁴ Fraction: 0.0001 · Denominator: 10,000
PRESENCE / ABSENCE METHOD

MPN Calculator

Estimate microbial concentration using replicate tubes.

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This is a maximum-likelihood estimate. It is not a replacement for validated MPN tables or your laboratory SOP.
Dilution ExponentPositive TubesTotal Tubes
MPN ESTIMATE — Enter tube results and calculate.
© Microbiology Notes | Laboratory Calculator
Educational tool. Verify results against your validated SOP.